PCR And Its Use

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of three-
temperatures: strand denaturation at 95degC, primer annealing at 55degC, and
primer extension at 72deg C, typically 30 seconds, 30 seconds, and 60 seconds
for the DNA Thermal Cycler and 4 seconds, 10 seconds, and 60 seconds for the
Thermal Cycler 9600, respectively."

Basically, that means that they set it to certain temperatures, then put it in
different cyles for different amounts of time. PCR machines can be compared
with washing machines. There are the different temperatures (here for example,
there is 72degC, where in the washing machine you would set it to cold/cold
respectively.

For it to properly replicate, we must know how to match each of the following:

A T G A T A T G G C A G C A A C G A C C A T A

the match would be

T A C T A T A C C G T C C T T G C T G T A T

The whole process is pretty much summed up like this: They heat up the DNA to
let the enzymes break it down (or 'unzip' its bonds). Then add specific amounts
of the primer (relative to the ...

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